Known as a club drug and date rape drug, GHB is a naturally occurring metabolite in the body. It inhibits a neurotransmitter called GABA, which reduces nerve activity in the central nervous system.
Recreational GHB can come in a white powder or clear liquid. The former is often dissolved into alcoholic drinks and given to people without them knowing. This drug is powerful enough to sedate victims, preventing them from defending themselves from sexual assault.
These victims may also be unable to remember the events. Aside from the facilitation of sexual assault, GHB is also used recreationally by many people who like to party or who enjoy the club scene. People intentionally take it to experience a euphoric high that enhances their experience at the club. A GHB high has also been marked by an increased sex drive.
The other results of the validation are described in Table 1. Due to the possibility of obtaining urine samples with high concentrations of GHB, dilution integrity was validated according to Guideline on bioanalytical method validation from European Medicine Agency. Subsequently, the extraction process was carried out with a calibration curve. The result obtained was 5. Figure 2 Chromatogram obtained through the analysis of GHB in urine samples according the purposed methodology.
Blank urine contains endogenous basal GHB. Therefore, it must be considered that GHB showed pharmacological effects like other central depressants drugs and rapid excretion less than 12 hours in urine samples.
These aspects could be responsible for the absence of real cases during the development of this method. This method is considered ultra-fast by the author, with time previously stipulated for agitation and centrifugation 5 minutes , derivatization 5 minutes and posterior chromatographic analysis 16 minutes.
The complete chromatographic runtime was 13 minutes, which is faster when compared to others in the literature with a full validation. LOQ values 0. So, the purpose method allows endogenous GHB determination when necessary. Several studies have evaluated the endogenous levels of GHB in a series of volunteers to determine a cut-off, differentiating endogenous from exogenous GHB. However, the urinary concentration of GHB after ingesting a dose of this drug 50 mg kg -1 was determined previously.
It was verified an extensive concentration range whose average was 3. So, this is an indication the detection period could be less than 12 hours after GHB administration. These procedures prevent in vitro GHB formation. Table 2 shows the scientific literature studies that deals with the analysis of GHB by GC-MS in urine samples and summarizes the total analysis time considering all steps, demonstrating that the purposed methodology adopted by the authors is more efficient in terms of time and handling.
In this regard, the procedure developed to determine this analyte in this biological matrix has a working range compatible with lower concentrations proposed by other studies without the need for changes. Therefore, this methodology can be used for clinical analysis when endogenous GHB requires to be measured.
Abrir menu Brasil. Abrir menu. Table 1 Confidence parameters of the validated method for the determination of GHB in urine samples sextuplicate for each point.
Acknowledgements We thank CNPq for the doctoral scholarship for carrying out this study. Mari, F. Andresen, H. Haller, C.
Elsohly, M. Drasbeck, K. Negrusz, A. Ferrara, S. LeBeau, M. Marsol-Vall, A. Villain, M. This is especially true if not actually alleging DFSA or another crime. Individuals arrested for driving under the influence who believe they may have been drugged, even if the allegation is made in a timely manner, also report difficulty in getting tests done for drugs like GHB.
The availability of an inexpensive field test can alter the landscape of response to drugging allegations dramatically. As with all drugs, screening tests are not the final word. Screening tests are important indicators of drugs being present. This is followed up with instrumentation tests to establish drug identity definitively and to identify the level of the drug s present.
As with all field test kits, they are merely indicators and would be followed up with definitive instrumentation testing in the lab. Vomit is also a potentially valuable source of drug evidence in sexual assault as well as death cases. Search this site:. You can order the date rape drug test at or order online. A drug test for GHB should be performed by a professional laboratory with appropriate licensing and experience with GHB testing.
Initial screening and confirmation testing should be included. We do not see GHB testing being available with oral fluid or saliva drug tests. So for GHB lab testing is always the best practice. Body weight, the frequency of use, and potency of the drug are all factors. With a urine test, the urine collection should occur no later than 24 to 48 hours after ingestion.
A hair test for GHB might be a better option. Hair should not be collected until days after the incident. The hair drug test for GHB is used to show use going back up to 90 days.
If you want to know how to pass a GHB drug test, it is best to stop using the drug. You can then always get a GHB drug test to make sure you are now clean. Often individuals awake after a night out with little memory of the events of the night before. Many times a date rape drug testing might be ordered to find out if you were drugged the night before. If you need to order a hair GHB drug test or a Date Rape drug test, call for for immediate service.
A comprehensive date rape drug test with urine or hair can also be ordered online.
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